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Reverse Phase HPLC

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I have resorted to reversed phase HPLC in order to monitor the decay of several pharmaceuticals during the application of Advanced Oxidation Processes (ozonation or electrochemical processes). So, this analytical technique allows me to carry out kinetic studies on the degradation of these products. Also the concentration of arising aromatic degradation intermediates was monitored using reverse phase HPLC.

 

compound reverse phase HPLC Analysis

paracetamol

paracetamol

hplc column Phenomenex Luna C18 5 μm (250 mm x 4.6 mm i.d.)

hplc reservoir Buffer KH2PO4 0.1 M + (tBu)4NHSO4 0.1 M + NaOH (until attaining pH 7.2) – ACN (70:30 v/v)

computer 0.8 mL min-1, room temperature.

hydroquinone

hydroquinone

 hplc column Phenomenex Luna C18 5 μm (250 mm x 4.6 mm i.d.)

hplc reservoir Buffer KH2PO4 0.1 M + (tBu)4NHSO4 0.1 M + NaOH (until attaining pH 7.2) – ACN (70:30 v/v)

computer 0.8 mL min-1, room temperature.

p-benzoquinone

p-benzoquinone

 hplc column Phenomenex Luna C18 5 μm (250 mm x 4.6 mm i.d.)

hplc reservoir Buffer KH2PO4 0.1 M + (tBu)4NHSO4 0.1 M + NaOH (until attaining pH 7.2) – ACN (70:30 v/v)

computer 0.8 mL min-1, room temperature.

chloroxylenol

chloroxylenol

 hplc column Thermo ODS hypersil 5 μm (150 mm x 3 mm i.d.)

hplc reservoir Buffer NH4H2PO4 / H3PO4 0.015 M (pH 3.0) – ACN (50:50 v/v)

computer 1.0 mL min-1, room temperature.

2,6-dimethyl-p-benzoquinone

2,6-dimethyl-p-benzoquinone

 hplc column Thermo ODS hypersil 5 μm (150 mm x 3 mm i.d.)

hplc reservoir Buffer NH4H2PO4 / H3PO4 0.015 M (pH 3.0) – ACN (50:50 v/v)

computer 1.0 mL min-1, room temperature.

ibuprofen

ibuprofen

 hplc column Thermo ODS hypersil 5 μm (150 mm x 3 mm i.d.)

hplc reservoir Buffer NH4H2PO4 / H3PO4 0.015 M (pH 3.0) – ACN (50:50 v/v)

computer 1.0 mL min-1, room temperature.

4-isobutyl-acetophenone

4-isobutylacetophenone

 hplc column Thermo ODS hypersil 5 μm (150 mm x 3 mm i.d.)

hplc reservoir Buffer NH4H2PO4 / H3PO4 0.015 M (pH 3.0) – ACN (50:50 v/v)

computer 1.0 mL min-1, room temperature.

diclofenac

diclofenac

 hplc column Thermo ODS hypersil 5 μm (150 mm x 3 mm i.d.)

hplc reservoir Buffer NH4H2PO4 / H3PO4 0.015 M (pH 3.0) – ACN (50:50 v/v)

computer 1.0 mL min-1, room temperature.

 

2,6-dichloroaniline

2,6-dichloroaniline

 hplc column Thermo ODS hypersil 5 μm (150 mm x 3 mm i.d.)

hplc reservoir Buffer NH4H2PO4 / H3PO4 0.015 M (pH 3.0) – ACN (50:50 v/v)

computer 1.0 mL min-1, room temperature.

 

 

 

Sunday, September 5th 2010

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