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Ion Exchange HPLC

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I have used Ion Exchange HPLC in order to monitor the concentration of several ions formed during the application of Advanced Oxidation Processes (ozonation or electrochemical processes) on pharmaceuticals. This analytical technique allows me to know the inorganic species the pharmaceutical molecule heteroatoms are transformed into after oxidation.

Here, experimental conditions I have used for the analysis of some inorganic ions are shown.

 

compound Ion Exchange HPLC Analysis

NH4+

hplc column Shodex IC YK-421 (125 mm x 4.6 mm i.d.) column.

hplc reservoir 5 mM tartaric acid, 1 mM dipicolonic acid, 24 mM boric acid.

computer 1.0 mL min-1, 40 ºC (104 ºF).

Cl-, NO2-, NO3-

 hplc column Shim-Pack IC-A1S (100 mm x 4.6 mm i.d.) column + Shim-Pack IC-GA1S (10 mm x 4.6 mm i.d.) guard column.

hplc reservoir 2.4 mM Tris(hydroxymethyl)aminomethane, 2.5 mM phtalic acid.

computer 1.5 mL min-1, 40 ºC (104 ºF).

NO3-, SO42-

(for solutions with high SO42- concentration)

 hplc column Shim-Pack IC-A1S (100 mm x 4.6 mm i.d.) column + Shim-Pack IC-GA1S (10 mm x 4.6 mm i.d.) guard column.

hplc reservoir 1.0 mM p-hydroxybenzoic acid, 1.1 mM N,N-diethyl ethanolamine.

computer 1.5 mL min-1, 40 ºC (104 ºF).

 

 

 

Sunday, September 5th 2010

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